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Bts minitube3/24/2023 ![]() ![]() The presence of insulin in human semen has been recently demonstrated, specifically in the sub-acrosomal region, the middle piece and the entire length of the flagellum (Aquila et al., 2005). Currently, the biggest challenge is to know the components that directly influence the metabolism of sperm cells, especially in swine. One of the main causes of the drop in fertility of stored boar semen is the decline of sperm metabolic energy (Ford & Waites, 1986), which is directly related to the amount of energy used. In this regard, several studies aimed at improving the quality of boar semen have been conducted (Vianna et al., 2004 Martins et al., 2009 Alvarenga et al., 2009). The determination of the sperm quality is essential, since infertility problems cause an increase of return to estrus rate and decreased birth rate, besides the reduction in average litter size (García et al., 2002). The technique of artificial insemination requires the use of high-quality semen, for better control and monitoring during processing (Waberski et al., 2008). ![]() In the latter aspect, artificial insemination becomes an important factor in improving the reproductive rates of a facility by reducing the concentration of sperm insemination doses and longer storage of diluted semen, optimizing the use of sires in the squad. The production of pig meat has been steadily tracking the growth of the world population and the development of this production depends on factors such as nutrition, health, management, genetics and reproduction. Key Words: artificial insemination, boar, hormone, metabolism, sperm, reproduction The addition of insulin at the mentioned concentrations does not influence the quality of insemination dose in pigs. There was a linear decrease in semen quality over storage time, regardless of insulin levels. The addition of insulin did not affect motility, sperm viability, the percentage of abnormal cells, the osmotic resistance or the degradation rate of motility in 120 minutes. Insulin used was NPH-human, added at the time of processing the doses. The experimental unit was made of two insemination doses of 100 mL each, with 3×10 9 spermatozoids. The experiment used sixteen ejaculates from four commercial breeding pigs, distributed in a randomized block design (ejaculate) with split plot along time (0, 24, 48 and 72 hours of storage) with four treatments (insulin levels - 0.0 4.0 8.0 and 12.0 IU per dose) and 16 repetitions. The objective of this study was to evaluate the effect of adding different doses of insulin to swine semen processed and stored at 15 ✬. Insulin addition to swine semen diluted and cooled at 15 ✬Įvandro César Pereira Cunha Márcio Gilberto Zangeronimo Luis David Solis Murgas Douglas Evangelista Braga Bárbara Azevedo Pereira Luiz Gustavo Pessoa Rocha Bruno Generoso Faria Luciano José Pereiraĭepartamento de Medicina Veterinária, Universidade Federal de Lavras, Caixa Postal 3037, 37200-000, Lavras, MG The addition of insulin at the mentioned concentrations does not influence the quality of insemination dose in pigs.Īrtificial insemination boar hormone metabolism sperm reproduction The experimental unit was made of two insemination doses of 100 mL each, with 3×10(9) spermatozoids. , Universidade Federal de Lavras, Departamento de Medicina Veterinária, Brazil About the authors , Universidade Federal de Lavras, Departamento de Medicina Veterinária, Brazil Luciano José Pereira , Universidade Federal de Lavras, Departamento de Medicina Veterinária, Brazil Bruno Generoso Faria , Universidade Federal de Lavras, Departamento de Medicina Veterinária, Brazil Luiz Gustavo Pessoa Rocha , Universidade Federal de Lavras, Departamento de Medicina Veterinária, Brazil Bárbara Azevedo Pereira , Universidade Federal de Lavras, Departamento de Medicina Veterinária, Brazil Douglas Evangelista Braga , Universidade Federal de Lavras, Departamento de Medicina Veterinária, Brazil Luis David Solis Murgas , Universidade Federal de Lavras, Departamento de Medicina Veterinária, Brazil Márcio Gilberto Zangeronimo ![]()
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